Effect of Cloxacillin Concentration in Ampiclox on the Suspectibility of some Clinical Bacteria

Synergistic antimicrobial effect of various ratios of ampicillin/cloxacillin combinations were investigated on susceptible clinical bacterial isolates of Staphylococcus aureus, Salmonella typhi, Escherichia coli and Proteus spp. Agar diffusion method was employed in the study to determine the potency of the varying ratios of ampicillin/cloxacillin combination. Variation in the zone diameters of inhibition were observed among the different varied ratios. In these case of ratio with higher concentration of cloxacillin, the zone diameter of inhibition is higher than those ratio with higher concentration of ampicillin. When cloxacillin and ampicillin were tested separately, cloxacillin was found to be very much effective than ampicillin especially against S. aureus ithat is why they are used synergistically. The study showed increase in the zone diameter of inhibition in all ratio with high concentration of cloxacillin, more than that of the present ratio of 250mg ampicillin and 250mg cloxacillin, but at statistical level, there was no significant different. Therefore, as such the present ratio 250mg ampicllin and 250mg cloxacillin is still considered as appropriate.